DNA

Part:BBa_K4765134:Design

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-10)


ribozyme connected: Dsup + FEN1 + H. ex mtSSB + SAHS 33020 + XRCC1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4573
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1886
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2464
    Illegal NgoMIV site found at 2782
    Illegal AgeI site found at 230
    Illegal AgeI site found at 301
    Illegal AgeI site found at 1570
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3415
    Illegal BsaI.rc site found at 227
    Illegal BsaI.rc site found at 3050
    Illegal BsaI.rc site found at 3131
    Illegal BsaI.rc site found at 5623
    Illegal SapI site found at 2944
    Illegal SapI.rc site found at 956


Design Notes

none


Source

synthetic

References